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. 2018 Jan 30;9:61. doi: 10.3389/fpls.2018.00061

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Copyright © 2018 Zuma, Dana and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Prolonged expression of InvINH1 delayed embryo growth. (A) The embryo phenotype of 21 individual T1 plants carrying pZOU-InvINH1 transgene. (B) The embryo phenotype of 20 individual T1 plants carrying pZou transgene. (C) Dissected embryos from the same silique of a T1 plant carrying pZOU-InvINH1. (D) Ectopic expression of InvINH1 in pZOU-InvINH1 transgenic plants. The InvINH1 mRNA level in whole siliques was determined by qRT-PCR at 5 dap. The expression level in wild-type (Col-0) siliques at 3 dap was used as a reference point (100%) to calculate the relative expression level in other samples. Around 50–60 seeds were dissected per line to determine the embryo phenotype. empty, undeveloped ovules; ebc, early-bent-cotyledon stage; mbc, mid-bent-cotyledon stage; lbc, late-bent-cotyledon stage (lbc). Bar = 500 μm.