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. 2018 Jan 30;9:12. doi: 10.3389/fgene.2018.00012

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Copyright © 2018 Guo, Dai, Li, Liu, Liu, Cheng, Cao, Chu, Zhai, Liu, Zhao and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction between GF14-JM22 protein and starch biosynthetic enzymes revealed by immunoblotting assays. M, Protein markers. (A) Gel image of SDS-PAGE. 1, Recombinant GF14-JM22 protein binding to S-protein agarose resin. 2, Blank control. 3, Recombinant GF14-JM22 protein through affinity chromatography. 4, Wheat amyloplast extracts. (B–F), Gel images of SH2-antibody, SSI-antibody, SSII-antibody, SBEIIa-antibody and SBEIIb-antibody, respectively. 1, Blank control. 2, Recombinant GF14-JM22 protein immunoblotting through affinity chromatography. 3, Wheat amyloplast extracts immunoblotting.