Figure 3.

TJ disruption plays a key role in lipid raft-mediated C. jejuni invasion of polarized epithelial cells. The number of intracellular bacteria was measured by gentamycin protection assay. (A) Caco-2 cells were treated with 4 mM EGTA treatment for 20 min followed by 10 mM MβCD for 1 h and were infected with C. jejuni for 6 h. (B) Caco-2 cells pre-treated as in (A) were infected followed with S. Enteritidis for 1 h. (C) Caco-2 cells pre-treated as in (A) were incubated with 0.2 mg/ml 4 kDa (white bar) or 10 kDa FITC-dextran for 2 h. FITC-dextran uptake was evaluated by fluorescence measurements. Results are shown as the mean ± SD; n = 4–6. Significant difference from the control or EGTA treated group are shown: N.S.; not significant; ^*P < 0.05; ^**P < 0.01.