Figure 5.

Fibrinogen-like protein 2 (Fgl2) deficiency promotes colitis-associated colorectal cancer (CAC). Fgl2^−/− mice (n = 7) and WT littermates (n = 8) were injected with azoxymethane (AOM) (10 mg/kg); 7 days later, mice were treated with three cycles of dextran sodium sulfate (DSS) (5 days of 1.8% DSS in water and 16 days of normal water). (A) Induction procedure for AOM/DSS-induced CAC in WT and Fgl2^−/− mice. (B) Body weight was monitored throughout the treatment regimen (n = 7–8 for WT, n = 6–7 for Fgl2^−/−). (C) Representative images of colorectal tumors (n = 7 for WT, n = 6 for Fgl2^−/−). (D) Number and size distributions of colorectal polyps (n = 7 for WT, n = 6 for Fgl2^−/−). (E) Average tumor burden (n = 7 for WT, n = 6 for Fgl2^−/−). Data are representative of three independent experiments. The statistical significance of differences was determined by two-way analysis of variance (B) with Bonferroni correction and unpaired two-tailed Student’s t-test (D,E). *P < 0.05, **P < 0.01.