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. 2018 Jan 30;6:3. doi: 10.3389/fchem.2018.00003

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Copyright © 2018 Fragkostefanakis, Kaloudas and Kalaitzis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression analysis of nine tomato prolyl 4 hydroxylases in different tomato seedling stages of 4, 6, 8, and 10 DPI (Days Post Imbibition). Relative expression was calculated based on the comparative Ct method using actin as internal standard. The Ct value for each gene was normalized to the Ct value for actin and was calculated relative to a calibrator (4 DPI) using the formula 2^−DDCt. Vertical bars are the average S.E. of three biological replicates. The ^*indicates significant difference in the gene's relative expression.