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. 2018 Jan 30;12:17. doi: 10.3389/fncel.2018.00017

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Copyright © 2018 Boido, De Amicis, Valsecchi, Trevisan, Ala, Ruegg, Hettwer and Vercelli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of muscular/NMJ morphology and agrin expression levels in the quadriceps of SMA mice. (A) Minimal Feret's diameter measured in P10 WT and SMA quadriceps. (B) Accumulation of neurofilament quantified by Imaris software and expressed as gray level. (C,D) Representative Western blots displaying full length (FL) agrin protein and the proteolytic fragment of 110 kDa in quadriceps of WT and SMA mice at P5 (C) and at P10 (D). Graphs below each image report the quantification of FL-agrin/GAPDH protein levels at P5 (E) and P10 (G), and the 110 kDa agrin fragment at P5 (F) and P10 (H). Unpaired T-test, ^*P < 0.05, ^***P < 0.001.