Table 1.
List of host proteomic studies of mycobacterial infection performed (A) on total cellular extracts, (B) on isolated cell organelles of infected cells, and (C) specifically on mycobacteria-containing vacuoles (MCVs) and bead-containing phagosomes.
| Reference | Type of sample | Experimental design | Peptide labeling | Proteins affected by infection | Main findings/pathways affected by mycobacterial infection |
|---|---|---|---|---|---|
| (A) Mass spectrometry (MS) data of total cellular host proteins | |||||
| Shui et al. (21) | Cellular extract | J774.A1 treated by Mycobacterium tuberculosis (Mtb) lipid extract | iTRAQ/SILAC | 166 | Immune response, oxidation and reduction, signal transduction, vesicle transport, metabolism, etc. |
| Yu et al. (22) | Lung tissue | Mtb-infected patient tissue, negative for HIV and HBV | Label-free | 6 Mtb peptides | Identification of novel Mtb antigens from granuloma lesions, antigen-specific IFN-γ secretion, and functional CTL responses |
| Kaewseekhao et al. (23) | Cellular extract/supernatant | THP-1 infected by Mtb H37Rv (MOI 1; 1–5 days) | Label-free | 283 | Cell cycle, antimicrobial and inflammatory responses, DNA replication, etc. |
| Li et al. (24) | Cellular extract | THP-1 infected by Mtb H37Rv/H37Ra or beads (MOI 35; 12 h) | TMT | 235 | Apoptosis, blood coagulation, and oxidative phosphorylation |
| Li et al. (25) | Cellular extract | THP-1 infected by BCG/Mycobacterium bovis/Mtb H37Rv (MOI 10; 24 h) | iTRAQ | 61 | Phagosome maturation and TNF signaling |
| (B) MS data of cell organelle proteins | |||||
| Wang et al. (26) | Cytoplasm and nucleus | RAW264.7 infected by Mycobacterium marinum (MOI 10; 4 h) | AACT/SILAC | 1,429 | Toll-like receptor 2-inflammatory responses, major histocompatibility complex-I processing and presentation, and transcriptional factors |
| Saquib et al. (27) | Endoplasmic reticulum | THP-1 infected by Mtb H37Rv/H37Ra (MOI 10; 12 h) | SILAC | 133 | Cytosolic Ca2+ levels, apoptosis, and cholesterol homeostasis |
| Diaz et al. (28) | Exosome | THP-1 infected by Mtb H37Rv (MOI 5; 24 h) | Biotinylated | 41 | Analysis of the exosome content |
| Kumar et al. (29) | Secretome | THP-1 infected by Mtb H37Rv/H37Ra/BND433/JAL2287 (MOI 10; 6–26 h) | iTRAQ | n/a | Analysis of the secretome |
| Kuo et al. (30) | Cell and plasma membrane | THP-1 derived DCs treated by heat-killed Mtb powder (10 µg/ml; 3 days) | Deuterium | 115 | Aminopeptidase N (ANPEP, CD13) |
| Long et al. (31) | Plasma membrane | THP-1 infected by BCG (MOI 5; 4 h) | SILAC | 559 | Immune interactions and lipid metabolism |
| (C) MS data of MCVs and phagosomes | |||||
| Rao et al. (32) | Phagosome | BMA.A3 infected by Mtb H37Rv/H37Rv ΔfbpA/BCG (MOI 5; 18 h) | Label-free | 322 | Phagosome maturation and role of the ER in phagosome biogenesis |
| Lee et al. (33) | Phagosome | THP-1 infected by BCG or beads (MOI 80; 3 h–5 days) | SYPRO Ruby | 447 | Membrane trafficking and signal transduction |
| Shui et al. (34) | Phagosome | RAW264.7 incubated with beads coated with ManLAM/PILAM/LPS | iTRAQ | 42 | Vesicle trafficking, phagosome maturation, and autophagosome |
| Li et al. (35) | Phagosome | BMDM and BMDC infected by Mtb H37Rv (MOI 5; 18 h) | Label-free | 41 | Phagosome maturation and antigen presentation |
| Herweg et al. (36) | Phagosome | RAW264.7 incubated with Mtb or trehalose-dimycolate-coated beads for 30 min | Label-free | 835 | Mitochondria, cytoskeleton, plasma membrane, ER, endosome, and Golgi-associated proteins |