Table 3.

Primers for the PCR-based SSR markers linked to RpsZS18 on soybean chromosome 2.

Markers	Primer sequence (5′−3′)	Position		Motif	Fragment	Expected
		Start	End		Size (bp)	χ2	P
ZCSSR8	F: CGAAGGAAGCCAAAGGAT	43310747	43310946	(GTG)7	200	0.59	0.75
	R: CCGCCTCACTGGCTTATT
ZCSSR10	F:AGGCGGGTGGTAGTTGTA	43312700	43312949	(AT)25	250	0.94	0.63
	R:TGGTAAATAGTAAAAGCA
ZCSSR28	F:GCAGAGAGAGACAAAGGGG	43366813	43367023	(AAG)5	211	1.18	0.56
	R:CCGTTTGCCATTCGTTGT
ZCSSR33	F:TAGTTGATAGCACCTGGGGACA	43377187	43377343	(TA)5	157	0.83	0.66
	R:TTCTCAGTCTCAAATGCC
Satt172	F:AGCCTCCGGTATCACAG	43443481	43443700	(AAT)19	220	1.52	0.47
	R:CCTCCTTTCTCCCATTTT
ZCSSR46	F:AAAGGGAGAAGCAAGTAAT	43522898	43523091	(GT)16	194	1.49	0.48
	R:TCGCAAACAGTAAACACG
BARCSOY-SSR_02_1562	F:CCCCCAAAGCGAAAAATAA	43656010	43656307	(TA)16	298	2.32	0.32
	R:CGATTCTATAATGGCGCTGTC
ZCSSR69	F:TTTTGGTCCATCAAGGGTA	43670051	43670293	(TC)6	243	3.35	0.19
	R:GCAAGCCATAGATAAGAA
ZCSSR130	F:TCCCCACAGTAACATAACA	44017408	44017605	(AC)10	198	3.36	0.19
	R:TTAGGCGTTTACTTCAGG
ZCSSR131	F:GAGTAAGATTGGGACAGA	44018687	44018852	(AC)24	166	3.17	0.21
	R:CTTTCTCATAAGCCATCT
Sat_183	F:GCGTCCAGCCTGACCATTTTA	44317044	44317314	(AT)28	271	2.46	0.30
	R:GCGTTCCAATGTCTGATTATTT
Satt274	F:GCGGGGTCAATTAGTTTTCGTCAGTT	45267040	45267222	(TAT)18	183	1.43	0.49
	R:GCGCACGGTATATAATCGAACCTAT