FIGURE 7.

UBR5 enhances cellular proliferation in T-cell leukemia/lymphoma cells. (A) 10³ stable (Left) Jurkat and (Right) SLB-1 lentiviral control or shUBR5 infected cells were plated in normal growth medium in 96-well plates and MTS assays were performed on triplicate wells at 24-h intervals for a total of 7 days. The average absorbance numbers are plotted and error bars denote SD. (Below) Immunoblot analysis was performed on total cell lysates to compare the levels of endogenous UBR5 and HBZ expression. β-actin was used as a loading control. (B) Cellular apoptosis was measured using a FITC Annexin V Apoptosis Detection Kit as described in the Section “Materials and Methods.” The percentage of cells undergoing apoptosis in lentiviral control infected cells was set at 1. The fold increase in apoptosis was measured in Jurkat and Jurkat-HBZ cells infected with lentiviral shUBR5. (Right) Immunoblot analysis was performed on total cell lysates to compare the levels of endogenous UBR5 and HBZ expression. β-actin was used as a loading control.