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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Cell Signal. 2017 Apr 28;36:98–107. doi: 10.1016/j.cellsig.2017.04.021

Fig. 3.

Fig. 3

The effect of C-loop mutations on basal and agonist-induced receptor binding. A. Basal BRET (net BRET ratio) and B. agonist-induced BRET change (delta BRET) between indicated luciferase-tagged receptors and Venus-tagged arrestin-3C-loop mutants was calculated, as described in the legend to Fig. 2. The cells were stimulated for 10 min with the appropriate agonist. BRET ratio obtained with negative control (arrestin-3 KNC [26,83] ) was subtracted. Means ± S.E. of at least three independent experiments are shown. Each experiment was performed in quadruplicate. Statistical significance was determined by one-way ANOVA: *, p < 0.05; **, p < 0.01; ***, p < 0.001, as compared with A87V base mutant binding to each receptor.