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. 2018 Jan 31;9:92. doi: 10.3389/fmicb.2018.00092

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Copyright © 2018 Vaidya, van den Bogert, Edwards, Boekhorst, van Gastelen, Saccenti, Plugge and Smidt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Principal component analysis (PCA) of the combined bacterial (16S rRNA gene), archaeal (16S rRNA gene) and anaerobic fungal (5.8S rRNA gene) qPCR data for rumen fluid (RF, Δ) and fibrous content (FC, aaa) samples. The GS100 diet has duplicate DNA extracts presented as individual datapoints. The percentages provided at the axes indicate the variation explained. (B) The corresponding loadings for the principal components indicate that anaerobic fungi are the major cause of sample separation in PC1, and archaea in PC2.