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(A) Volume of the astrocytes in the hippocampus in SAH and sham groups 4 days following the surgery on GFAP immunostained sections. (B) Illustration of the 3D nucleator tool for the measurment of the volume of GFAP positive astrocytes in the hippocampus. The intersections between six half-lines (blue half-lines) and the border of the cell soma were specified by using a 100× objective. Scale bar is 20 μm; ∗∗∗p = 0.001. Scale bar = 10 μm.
