Figure 2.

PRIMA‐1 preferentially induces apoptosis in esophageal squamous cell carcinoma (ESCC) cell lines with a TP53 missense mutation. A, Fluorence‐activated cell sorting plots of Annexin V/propidium iodide‐labeled cells treated with 25 and 50 μmol/L PRIMA‐1 for 24‐48 h. B, Percentage of Annexin V‐positive cells after treatment with PRIMA‐1 using flow cytometry; n = 3‐5. Error bars show the standard deviation. P‐values refer to the comparison of PRIMA‐1‐treated samples with untreated samples within each cell line. **P < .01, ***P < .001