Figure 3.

Helicobacter pylori decreased microRNA (miR)‐212‐3p and miR‐361‐3p expression. ET‐1A and OE33 cells were cocultured with H. pylori (100 MOI) for 12 h, relative expression levels of miR‐212‐3p (A) and miR‐361‐3p (B) were analyzed by real‐time quantitative PCR. C, HET‐1A cells were exposed to H. pylori extract (HPE) for 36 wk and relative expression levels of miR‐212‐3p and miR‐361‐3p were determined. In Barrett's esophagus patients with or without H. pylori esophageal colonization, the expression of miR‐212‐3p (D), miR‐361‐3p (E), COX2 (F), and caudal‐type homeobox protein 2 (CDX2) (G) were detected. All miRNA expression was normalized to U6 small nuclear RNA. COX2 and CDX2 mRNA expression was normalized to GAPDH. Data are expressed as mean ± SD. *P < .05. NS, not significant. All results shown are representative of three independent experiments