Figure 4.
Prophylaxis effect of tumor vaccine modified with LX/IL(15+7). Mice were immunized with irradiated B16, B16‐LX/RFP or B16‐LX/IL(15+7) twice with 1‐week intervals, respectively, then mice were challenged with 1 × 105 B16‐F10 cells (A) or 1 × 105 EL‐4 cells (B) 2 weeks later. The tumor volumes were monitored daily. Mice were killed on day 7 post‐tumor challenge; the total numbers of splenocytes and frequencies of total T, CD4+ T, CD8+ T and NK cells in the spleen (C) and tumor‐infiltrating lymphocytes (TIL) (D) were analyzed. The experiments were performed with 6 mice per group. The data shown are representative of the 3 experiments. *P < .05 and **P < .01