Figure 2.

Immunodetection of binding partners of PRDM14. A, Eluents of a Halo pull‐down assay were analyzed by Wes using anti‐PRDM14, anti‐HSP90α, anti‐GRP78, anti‐γ‐Catenin, and anti‐caspase‐14 antibodies. B, C, Co‐immunoprecipitation (Co‐IP) experiments were carried out with lysates prepared from Halo‐PRDM14‐transduced HCC1937 and MDA‐MB231 cells. The lysates were immunoprecipitated with anti‐HSP90α, anti‐GRP78, or anti‐γ‐Catenin antibodies and then immunoblotted (IB) with (B) each antibody and (C) anti‐Halo antibody. GRP78, glucose‐regulated protein 78; HSP, heat shock protein