Figure 3.
Binding and affinity analysis by surface plasmon resonance. A, Sensorgrams of the proteins (10 μg/mL) binding to surface GST‐PRDM14. B, Different concentrations of HSP90α and glucose‐regulated protein 78 (GRP78) (Conc 1‐5) flowed over GST‐PRDM14 on the anti‐GST‐immobilized sensor chip and analyzed by single‐cycle kinetic analysis. KD, dissociation constant. C, Schematic representation of PRDM14 full length and PRDM14‐ΔC. PRDM14 protein has a PR/SET domain (aa 266‐367) and six zinc finger motifs (aa 402‐426, aa 434‐453, aa 463‐483, aa 491‐511, aa 519‐537, and aa 548‐565). D, Different concentrations of HSP90α and GRP78 flowed over GST‐PRDM14‐ΔC on the anti‐GST‐immobilized sensor chip, analyzed by single‐cycle kinetic analysis. GRP78, glucose‐regulated protein 78; HSP, heat shock protein