Table 2. Vaccine formulations, number of immunization and mean body temperature of cow post challenge-exposure.
| Group | Vaccine formulations | Number of immunization | Inoculum strain c) | Mean body temperature post challenge-exposure (°C) d) |
|||||
|---|---|---|---|---|---|---|---|---|---|
| Day 0 | Day 1 | Day 2 | Day 3 | Day 4 | Day 5 | ||||
| 1 | 50 µg each rOmpH/rLKT + CpG ODN 10 µg a) | 5 | M-1404 | 37.06 ± 0.04 | 37.63 ± 0.04 | 37.42 ± 0.02 | 37.26 ± 0.03 | 36.84 ± 0.02 | 36.59 ± 0.01 |
| 2 | 100 µg each rOmpH/rLKT + CpG ODN 10 µg a) | 5 | 37.06 ± 0.03 | 37.64 ± 0.02 | 37.44 ± 0.01 | 37.36 ± 0.03 | 36.97 ± 0.03 | 36.83 ± 0.04 | |
| 3 | Commercial hemorrhagic septicemia vaccine b) | 2 | 37.12 ± 0.04 | 37.54 ± 0.03 | 37.52 ± 0.03 | 37.37 ± 0.02 | 37.06 ± 0.02 | 37.27 ± 0.02 | |
| 4 | No vaccination | 2 | 37.04 ± 0.03 | 38.54 ± 0.01e | 38.03 ± 0.02 | 37.49 ± 0.02 | 37.03 ± 0.03 | 37.10 ± 0.02 | |
a) Intranasal administration with 100 µl/dose, b) Intramuscular administration with 1.0 ml/dose. c) Challenge inoculum was 1 ml of PBS containing approximately 103 CFU/ml. d) Daily average body temperature. e) Experiment was terminated due to the clinical signs of cow.