Fig. 4.

Determination of VP1/2A cleavage by IF staining for FMDV proteins within cells. FMDV proteins within uninfected or FMDV-infected cells (using m.o.i. of 0.1) were detected (at 6 h p.i.) using an anti-FMDV A-Iraq polyclonal antibody or an anti-2A antibody (as indicated) and a secondary antibody labelled with Alexa Fluor 568 (red) as in Fig. 3. The codon for residue 210 within VP1 (in parentheses) and the resulting individual amino acid residue within the different rescued viruses are indicated. Uninfected cells were used as a negative control. The cellular nuclei were visualized with DAPI (blue). Bar, 200 µm.