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. 2018 Jan 12;19(2):368–381. doi: 10.15252/embr.201745225

Figure EV2. HSET levels control the timing of spindle bipolarization.

Figure EV2

  1. Histogram showing the mean time of bipolarization setup (data are represented as mean ± SD). Bipolarity was scored when two poles were distinguishable. The mean time of bipolarization setup for oocytes overexpressing HSET WT is 1 h and 19 min (blue bar), compared to 4 h and 3 min for controls (gray bar), ***P‐value < 0.0001, compared to 6 h and 55 min for oocytes inhibited for HSET (purple bar), ***P‐value < 0.0001. Statistical significance of differences is assessed with a Mann–Whitney test.
  2. Histogram showing the mean time of bipolarization setup in controls vs. HSET N593K expressing oocytes (data are represented as mean ± SD). Bipolarity was scored when two poles were distinguishable. The mean time of bipolarization setup for oocytes overexpressing HSET N593K is 2 h and 36 min (dark blue bar), compared to 3 h and 10 min for the controls (gray bar). Statistical significance of differences is assessed with a t‐test: *P‐value = 0.034.
  3. Graph representing the kinetics of spindle bipolarization in controls (gray) vs. oocytes inhibited for HSET with AZ82 (purple) or CW069 (violet). The number of oocytes analyzed is written in parentheses.
  4. Histogram showing the mean time of bipolarization setup. Bipolarity was scored when two poles were distinguishable. Data are represented as mean ± SD. The mean time of bipolarization setup for controls (gray bar) is 4 h and 18 min, compared to 6 h and 55 min for oocytes inhibited for HSET with AZ82 (purple bar), compared to 7 h and 0 min for oocytes inhibited for HSET with CW069 (violet bar). Statistical significance of differences is assessed with a Mann–Whitney test: **P‐value = 0.007, ***P‐value < 0.0001, not significant (n.s.) P‐value = 0.929. The number of oocytes analyzed is written in parentheses.