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. 2018 Jan 12;19(2):290–304. doi: 10.15252/embr.201744296

Figure 1. Myo1C constantly shuttles between the nucleus and cytoplasm.

Figure 1

  1. Fluorescence recovery after photobleaching experiments with GFP‐Myo1C, GFP‐actin, and GFP; data represent nuclear fluorescence levels normalized to the value before bleaching and are the mean ± SD (GFP n = 10; GFP‐actin n = 8; GFP‐Myo1C n = 11).
  2. Nuclear import rate derived from the FRAP curves; data represent mean rates from individual experiments ± SD (n as in A).
  3. FLIP experiments with GFP‐Myo1C and GFP‐Myo1C tail WT; data represent normalized nuclear fluorescence levels and are the mean ± SD (n = 8).
  4. Nuclear export rate quantified from FLIP curves; data represent mean rates from individual experiments ± SD (n = 8).
  5. Western blot showing that GFP‐Myo1C is expressed as a full‐size protein with no signs of degradation.
  6. Schematic of Myo1C constructs used in the present study. Pleckstrin homology domain of Myo1C (PH), chicken pyruvate kinase (PK), and pleckstrin homology domain of phospholipase C, isoform δ (PH‐PKCδ), are indicated in the chart. HA‐tag was in the N‐terminus of the constructs. Note that “tail” construct contains also the neck region of Myo1C.