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. 2018 Jan 12;19(2):290–304. doi: 10.15252/embr.201744296

Figure 5. In vitro import assay shows that association of Myo1C with ER precedes its nuclear import.

Figure 5

  1. First, cells were incubated with import substrate (GFP‐Myo1C tail WT) in the absence of energy to saturate ER with the myosin (“− energy”). Then, unbound substrate was washed off and cells were supplemented with energy mix (creatine kinase, phosphocreatine, GTP, ATP), HeLa cytosolic extract, and incubated for 30 min at 30°C. Scale bar, 20 μm.
  2. A higher‐magnification image showing that recombinant GFP‐Myo1C tail WT associated with the membranes of the endoplasmic reticulum in digitonin‐permeabilized HeLa cells. Scale bar, 10 μm.