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. 2018 Feb 5;131(3):323–329. doi: 10.4103/0366-6999.223840

Figure 3.

Figure 3

Nestin gene and protein expressions were significantly upregulated in the condition of antivenom with HBO at 1 h after injection Deinagkistrodon acutus venom. (a) The histogram was the quantified PCR of Nestin gene expression. The antivenom with HBO significantly upregulated the Nestin gene expression, especially at 1 h after injection of snake venom. (b) Changes in expression level of nestin protein by Western blotting assay. (c) Semiquantitative results from the Western blotting assay in (b). The density of nestin band was normalized against beta-actin. Values were normalized to control and expressed as the mean ± standard deviation (n = 3). *P < 0.05: Compared with control at the same time point; †P < 0.05: Compared with the snake venom group at the same time point; ‡P < 0.05: Compared with the antivenom group at the same time point; HBO: Hyperbaric oxygen; PCR: Polymerase chain reaction.