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. 2018 Feb 5;17:17. doi: 10.1186/s12934-018-0868-0

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Chimeric VLP isolated from strain D#79 composed of dS and E2CSFV102-dS were analyzed under native conditions by N-SIM. Two series of images obtained from the same sample are presented showing fluorescence immunolabeling of dS in green (a-1, a-2), CSFV E2 antigen in red (b-1, b-2) and co-localization of the two labels in superimposed images in yellow (c-1, c-2). In each series of images two spots were consistently marked by arrows: signals of the size expected for individual VLP (white); largest signals in the respective frame (green)