Table 2.
Summary of fusion proteins constructed and recombinantly produced in H. polymorpha
| Viral source protein (Genbank accession number) | aa used for fusion protein (number of predicted N-glycosylation sitesa) | Fusion protein designation | C- or N-terminally fused to dS | Number of aab | MW [kDa] | Signal sequence (CL) or start methionine (M) included | Genbank accession number for the fusion protein coding gene |
|---|---|---|---|---|---|---|---|
| BVDV E2 (AEV54362.1) | 1–344 (4) | E2BVDV344-dS | N | 511 | 57.5 | CL | MG280712 |
| 1–196 (2) | E2BVDV196-dS | N | 363 | 40.3 | CL | MG280713 | |
| 1–196 (2) | dS-E2BVDV196 | C | 363 | 40.3 | M | MG280714 | |
| CSFV E2 (AAT85717.1) | 1–337 (5) | E2CSFV337-dS | N | 504 | 56.1 | CL | MG280715 |
| 1–184 (2) | E2CSFV184-dS | N | 351 | 38.7 | CL | MG280716 | |
| 1–102 (0) | E2CSFV102-dS | N | 270 | 29.5 | M | MF510123 | |
| FeLV env (AAA43051.1) | 1–412 (9) | FeLVp45-dS | N | 580 | 63.7 | M | MG280717 |
| 1–132c (0) | FeLVp15E-dS | N | 326 | 35.8 | M | MG280718 | |
| WNV E (ADI33161.1) | 293–454 (0) | EDIIIWNV-dS | N | 330 | 35.3 | M | MG280719 |