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. 2018 Feb 5;17:18. doi: 10.1186/s12934-018-0865-3

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Western blotting was performed with the total protein isolated from WT and transformed Mag II-CB (a) and CB (b) C. militaris. Lane 1: WT C. militaris. Lanes 2–7: Transformed Mag II-CB (a) and CB (b) C. militaris. M denotes the protein marker. Coomassie blue-stained SDS-PAGE products showing Mag II-CB (c) and CB (d) proteins expressed by transformed C. militaris (lane 1) and the purified samples (lane 2). Western blotting analysis of the Mag II-CB (e) and CB (f) protein in WT C. militaris (lane 1), transformed C. militaris (lane 2) and the purified samples (lane 3)