Effect of free radical scavenger on Gene Expression: T24 and 253J cells were exposed to BCG. Post BCG exposure, gene expression of various genes was measured using q-RT PCR. Treatment groups included untreated, ebselen treated, BCG treated and BCG with ebselen. BCG exposure resulted in significant increased the expression of 8 immune response genes as measured by qRTPCR in both cell lines (ANOVA, p < 0.05). Ebselen in combination with BCG reduced UC cell expression of all measured genes with 7 of 8 and 6 of 8 reaching the level of statistical significance in 253J and T24 cells respectively (ANOVA, P < 0.05).