UA induces the suppression of tumor growth of BGC823-bearing nude mice. BGC823 cells were injected into the right flank of 5-week-old male nude mice. After injection of BGC823 cells, nude mice were treated with PBS, 5-FU (25 mg/kg), and UA (100 mg/kg) for 11 days. (A) Body weight changes of the mice during 11 days of treatment. # P<0.05 (UA compared with 5-FU), ** P<0.01 (control compared with 5-FU) (B) The tumor size of the xenograft tumors („Materials and methods” section). # P<0.05, ## P<0.01 (UA compared with 5-FU), * P<0.05, ** P<0.01 (UA compared with control). (C) Tumor weight changes of the mice during 11 days of treatment. * P<0.05, ** P<0.01, *** P<0.001. (D) The image of tumors. (E–G) Tumor tissues were examined by H&E staining (E, yellow arrow indicates necrotic cells) and immunohistochemical staining with Bax (F) and Bcl2 (G) antibodies. Original magnifications: 200×. (H, I) The mean value of the H-score of Bax (H) and Bcl2 (I). * P<0.05, ** P<0.01, *** P<0.001. All data represent the mean ±SD.