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. 2017 Dec 18;293(5):1536–1549. doi: 10.1074/jbc.RA117.000125

Table 1.

Peptides used in this study

Peptide Amino acid sequence Theoretical massa Experimental massb Purityc ACN gradientd Retention time ACNe
Da Da % min %
Ctnf KRFKKFFKKVKKSVKKRLKKIFKKPMVIGVTIPF-amide 4151.39 4152.45 98 15–40 9.556 30.9
RhB-Ctn RhB-KRFKKFFKKVKKSVKKRLKKIFKKPMVIGVTIPF-amide 4576.94 4575.97 99 20–40 10.997 34.7
Ctn(15–34) KKRLKKIFKKPMVIGVTIPF-amide 2371.11 2371.23 99 10–40 10.131 30.3
RhB-Ctn(15–34) RhB-KKRLKKIFKKPMVIGVTIPF-amide 2796.66 2795.90 98 20–40 10.670 34.2

a Theoretical molecular mass was calculated using GPMAW version 8.10.

b Experimental molecular mass was determined from the MS spectra shown in Fig. S1b.

c Peptide purity was estimated by peak integration of the analytical HPLC chromatograms shown in Fig. S1a.

d ACN gradient over 15 min used to run the analytical HPLC. Range indicates the initial and final ACN concentrations.

e Percentage of ACN corresponding to the eluted peptide, calculated from the gradient used and the retention time.

f Protein Data Bank accession code of Ctn: 2MWT.