Table 1.

Peptides used in this study

Peptide	Amino acid sequence	Theoretical massa	Experimental massb	Purityc	ACN gradientd	Retention time	ACNe
		Da	Da	%		min	%
Ctnf	KRFKKFFKKVKKSVKKRLKKIFKKPMVIGVTIPF-amide	4151.39	4152.45	98	15–40	9.556	30.9
RhB-Ctn	RhB-KRFKKFFKKVKKSVKKRLKKIFKKPMVIGVTIPF-amide	4576.94	4575.97	99	20–40	10.997	34.7
Ctn(15–34)	KKRLKKIFKKPMVIGVTIPF-amide	2371.11	2371.23	99	10–40	10.131	30.3
RhB-Ctn(15–34)	RhB-KKRLKKIFKKPMVIGVTIPF-amide	2796.66	2795.90	98	20–40	10.670	34.2

^a Theoretical molecular mass was calculated using GPMAW version 8.10.

^b Experimental molecular mass was determined from the MS spectra shown in Fig. S1b.

^c Peptide purity was estimated by peak integration of the analytical HPLC chromatograms shown in Fig. S1a.

^d ACN gradient over 15 min used to run the analytical HPLC. Range indicates the initial and final ACN concentrations.

^e Percentage of ACN corresponding to the eluted peptide, calculated from the gradient used and the retention time.

^f Protein Data Bank accession code of Ctn: 2MWT.