Figure 3.

Angptl2 deficiency in skeletal myocytes enhances satellite cell activity. a, relative transcript levels of satellite cell activation-associated genes (Cd34, Pax3, Pax7, Myf5, MyoD, and myogenin) and skeletal muscle myosin subtypes (Myh1, Myh2, Myh4, and Myh7) in musculus gastrocnemius and musculus soleus of Angptl2^Flox/Flox;MCK-Cre or Angptl2^Flox/Flox mice (n = 4 per group). b, representative immunostaining and quantifications of CD34 (green) and PAX7 (red) in musculus gastrocnemius of Angptl2^Flox/Flox;MCK-Cre or Angptl2^Flox/Flox mice. Myofibers are co-stained with DAPI (blue) and WGA (gray). Yellow arrowheads in Merge indicate CD34- and PAX7-positive cells. Red arrowheads in Merge indicate PAX7-positive cells. (Scale bar, 50 μm. Six different fields are quantified per mouse, n = 3 per group.) c, representative Western blot and PAX7 quantification in musculus gastrocnemius and musculus soleus of Angptl2^Flox/Flox;MCK-Cre and Angptl2^Flox/Flox mice. Hsc70 serves as the internal loading control. Values in Angptl2^Flox/Flox mice were set to 1 (a and c). All data are presented as means ± S.D. (a and b) or means ± S.E. (c); statistical significance was determined by Student's t test. *, p < 0.05; **, p < 0.01.