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. 2017 Nov 30;293(5):1596–1609. doi: 10.1074/jbc.M117.814996

Figure 5.

Figure 5.

Angptl2-deficient skeletal myocytes show decreased inflammation and ROS levels and increased satellite cell activity. a, c, and d, relative expression of Il-1β, Il-6 (a), catalase (c), and satellite cell activation-associated (d) mRNAs in denervated musculus gastrocnemius of Angptl2Flox/Flox;MCK-Cre or Angptl2Flox/Flox mice (n = 5 per group). Relative mRNA expression was normalized to 18S mRNA. b, representative Western blot of 4-HNE-adducted proteins in denervated musculus gastrocnemius of Angptl2Flox/Flox;MCK-Cre or Angptl2Flox/Flox mice. Asterisks indicate decreased levels of these proteins seen in Angptl2Flox/Flox;MCK-Cre mice. e, representative Western blot and PAX7 quantification 14 days after denervation in musculus gastrocnemius of Angptl2Flox/Flox;MCK-Cre or Angptl2Flox/Flox mice. Hsc70 and CBB serve as internal loading controls (b and e). Values at day 0 of denervation in both Angptl2Flox/Flox;MCK-Cre and Angptl2Flox/Flox mice (a, c, and d) and in Angptl2Flox/Flox mice (e) were set to 1. All data are presented as means ± S.E. (a, c, and d) or means ± S.D. (e); statistical significance was determined by Student's t test. *, p < 0.05; **, p < 0.01.