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. 2017 Dec 12;293(5):1610–1622. doi: 10.1074/jbc.M117.801480

Figure 7.

Figure 7.

ST6Gal-I inhibits TNF-induced apoptosis. A, MiaPaCa-2 EV and KD cells were treated with TNF for 6 and 24 h and immunoblotted for cleaved caspase-8 (cl. caspase 8). The β-tubulin blot is a duplicate of the β-tubulin blot shown in Fig. 6A because the data from Figs. 6A and A are from the same experiment. B, MiaPaCa-2 cells were treated with TNF for 8 and 24 h and immunoblotted for cleaved caspase-3 (cl. caspase-3). C, MiaPaCa-2 cells were treated with TNF for 4 and 24 h and analyzed via a luminescence assay that detects caspase-7 and -3 activity. A representative experiment performed in quadruplicate is shown; data are plotted as mean ± S.D. D, OV4 EV or OE cells were treated with TNF plus CHX for 3, 6, and 24 h and immunoblotted for cleaved caspase-8. E, OV4 cells were treated with TNF plus CHX for 24 h and immunoblotted for cleaved caspase-3. F, OV4 cells were treated with TNF plus CHX for 4 and 24 h and analyzed via a luminescence assay for caspase-7 and -3 activity. A representative experiment in quadruplicate is shown; data are plotted as mean ± S.D.