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. 2017 Nov 20;293(5):1651–1665. doi: 10.1074/jbc.M117.811521

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Figure 3. — Physical interaction of TFIIB_c and Ssu72. A, ITC experiment of Ssu72 and TFIIBc. The best fit K_d value (95% confidence interval) is 20.2 (16.8–24.4) μm. B, FLAG pulldown assay with TFIIB-FLAG variants and His₆-Ssu72, as visualized by Coomassie staining and Western blotting with a His₆ antibody. *, IgG light chain that leeched from the resin upon elution with glycine. C, FLAG pulldown assay with TFIIBc-FLAG mutants and His₆-Ssu72. D, FLAG pulldown assay with TFIIBc-FLAG WT and mutants from various species (top labels) with His₆-Ssu72 from the corresponding species as visualized by Western blotting with a His₆ antibody. In the figure, h represents human, d is D. melanogaster, and y is S. cerevisiae. Lanes labeled with FLAG indicate controls without TFIIBc added.