Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 20;293(5):1651–1665. doi: 10.1074/jbc.M117.811521

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 6. — Disruption of TFIIB autoinhibition by Ssu72. A, competition cross-linking between SUMO TFIIBn W52pBpA and Ssu72 for TFIIBc WT visualized by anti-FLAG Western blotting. Lanes labeled Mut utilized the TFIIBc K189E/R193E mutant. Bottom, TFIIBc-FLAG becomes distorted in some lanes due to GST that runs in a position similar to that of TFIIBc. B, competition cross-linking assay between Ssu72 C131pBpA and either GST-TFIIBn or GST-VP16AD for TFIIBc as visualized by FLAG antibody Western blotting.