Table 1.

Metabolic parameters of wildtype and VDAC3-KO mice

Week 0 are baseline measurements before special diet; Week 2 is after 2 weeks on normal-salt, low-salt, high-salt diet, or high-salt diet plus benzamil (see “Experimental procedures”). Values are mean ± S.D. Values in parentheses are number of mice.

Parameter	Week	Wildtype				VDAC3-KO
		Normal diet	Low-salt	High-salt	High-salt plus benzamil	Normal diet	Low-salt	High-salt	High-salt plus benzamil
Food intake	0	2.18 ± 0.450 (4)	2.23 ± 0.450 (5)	2.82 ± 1.220 (5)	2.56 ± 0.593 (5)	2.80 ± 1.031 (3)	2.38 ± 0.453 (4)	2.68 ± 0.993 (4)	2.52 ± 0.697 (4)
(g/day)	2	3.11 ± 0.444 (4)	2.86 ± 0.760 (5)	2.74 ± 0.481 (5)	2.50 ± 0.279 (5)	3.43 ± 0.083 (3)	3.18 ± 0.385 (4)	3.25 ± 0.487 (4)	2.50 ± 0.429 (4)
Water intake	0	3.24 ± 0.250 (4)	3.46 ± 0.788 (5)	3.92 ± 1.588 (5)	3.48 ± 0.648 (5)	3.86 ± 0.605 (3)	3.33 ± 0.393 (4)	3.67 ± 1.198 (4)	4.64 ± 1.877 (4)
(ml/day)	2	3.84 ± 0.783 (4)	2.64 ± 0.714 (5)	9.09 ± 1.373 (5)	7.69 ± 1.660 (5)	3.88 ± 1.093 (3)	2.73 ± 1.172 (4)	8.52 ± 0.857 (4)	6.91 ± 0.971 (4)
Body weight	0	19.2 ± 0.206 (4)	18.5 ± 3.243 (5)	20.5 ± 2.098 (5)	19.8 ± 3.935 (5)	20.7 ± 2.803 (3)	19.3 ± 1.865 (4)	21.3 ± 3.629 (4)	18.8 ± 2.051 (4)
(g)	2	20.2 ± 1.251 (4)	18.6 ± 2.348 (5)	19.8 ± 2.166 (5)	19.4 ± 3.871 (5)	21.0 ± 1.665 (3)	19.6 ± 1.746 (4)	21.4 ± 3.981 (4)	18.7 ± 1.652 (4)
Urine output	0	0.59 ± 0.278 (4)	1.01 ± 0.358 (5)	0.85 ± 0.48 (4)	0.76 ± 0.411 (5)	1.10 ± 0.377 (3)	0.58 ± 0.222 (4)	1.11 ± 0.539 (4)	0.90 ± 0.337 (4)
(ml/day)	2	0.66 ± 0.138 (4)	0.44 ± 0.219 (5)	5.44 ± 0.841 (5)	3.98 ± 0.87 (5)	1.07 ± 0.115 (3)	0.30 ± 0.135 (4)	5.39 ± 0.557 (4)	2.88 ± 0.512 (4)
Urine osmolality	0	4153 ± 741.9 (4)	3804 ± 361.5 (5)	4746 ± 1015 (5)	4370 ± 548.9 (5)	4053 ± 507.2 (3)	5425 ± 984.3 (4)	4250 ± 394 (4)	5010 ± 730.8 (4)
(mmol/kg·H2O)	2	4575 ± 346.7 (4)	4250 ± 1255 (5)	1471 ± 169.9 (5)	1739 ± 123.7 (5)	4023 ± 486.4 (3)	4748 ± 1107 (4)	1628 ± 239.5 (4)	2014 ± 330.3 (4)
Urine Na+	0	0.08 ± 0.023 (3)	0.16 ± 0.08 (4)	0.17 ± 0.142 (3)	0.16 ± 0.114 (4)	0.18 ± 0.097 (3)	0.12 ± 0.063 (4)	0.21 ± 0.106 (4)	0.18 ± 0.079 (4)
(mmol/day)	2	0.16 ± 0.047 (3)		2.91 ± 0.336 (4)	2.64 ± 0.376 (4)	0.20 ± 0.019 (3)		3.10 ± 0.216 (4)	2.04 ± 0.303 (4)
Urine K+	0	0.18 ± 0.020 (3)	0.39 ± 0.123 (4)	0.36 ± 0.271 (3)	0.36 ± 0.210 (4)	0.43 ± 0.148 (3)	0.23 ± 0.086 (4)	0.50 ± 0.218 (4)	0.43 ± 0.155 (4)
(mmol/day)	2	0.36 ± 0.085 (3)	0.17 ± 0.080 (4)	0.43 ± 0.046 (4)	0.39 ± 0.038 (4)	0.48 ± 0.031 (3)	0.11 ± 0.059 (4)	0.45 ± 0.062 (4)	0.30 ± 0.032 (4)
Na/K ratio	0	0.46 ± 0.123 (3)	0.41 ± 0.089 (4)	0.43 ± 0.063 (3)	0.44 ± 0.079 (4)	0.41 ± 0.086 (3)	0.51 ± 0.118 (4)	0.42 ± 0.057 (4)	0.40 ± 0.068 (4)
	2	0.43 ± 0.034 (3)		6.82 ± 0.347 (4)	6.77 ± 0.433 (4)	0.42 ± 0.064 (3)	—	6.64 ± 0.280 (3)	6.86 ± 0.405 (3)