Representative traces of currents recorded during stimulation with low pH and GMQ. Currents recorded from CHO cells expressing mASIC3 (A), rASIC3 (B), or nmrASIC3 (C) stimulated with either pH 4.0 or 3.0 solution, and stimulated with 1 mm GMQ (D–F). G, Western blot of whole cell lysates (input) and biotinylated surface fraction from cells transfected with mASIC3, nmrASIC3, or co-transfected with m/nmrASIC3 and rASIC2a and stained with anti-ASIC3 antibody, anti-ASIC2 antibody, or anti-β-Actin antibody.