Multiple sequence alignment of rodent and human ASIC3 protein including species closely related to naked mole-rat or living in similarly hypoxic/hypercapnic habitats.
A, amino acids 1–10, methionine is missing in the naked mole-rat (arrow). B, amino acids 55–69, change of alanine 62 to glutamate in naked mole-rat (arrow). C, amino acids 99–112, change of arginine to histidine in naked mole-rat and Damaraland mole-rat (arrow). D, pH-response curves of mASIC3 and mASIC3-ATG. E, pH-response curves of rASIC3, rASIC3-A62E, rASIC3-A62E/R102H. Data were analyzed by a t test.