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. 2017 Dec 8;293(5):1767–1780. doi: 10.1074/jbc.M117.809608

Figure 2.

Figure 2.

In vitro maturation of COCs classified by BCB staining. A, morphology of COCs (IVM for 24 h) and denuded oocytes (IVM for 44 h), as well as representative images of live (a), MII (b), and dead oocytes (c). Scale bar, 200 μm for the first and second rows. 50 μm for a–c. Black arrows, MII oocytes with the first polar body; gray arrows, dead oocytes with broken ooplasmic membrane. B, percentages of oocytes matured to MII stage, or died during IVM. C, representative images of parthenotes cleaved and developed to blastocysts. Scale bar, 200 μm. D, percentages of parthenotes cleaved and developed to blastocysts for BCB-control, BCB-negative, and BCB-positive groups. E, representative images of Hoechst 33342-stained blastocysts. Scale bar, 200 μm. F, average cell number per blastocyst. **, p < 0.01. ***, p < 0.001.