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. 2017 Dec 8;293(5):1767–1780. doi: 10.1074/jbc.M117.809608

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Figure 5. — CDC5L's function in meiotic maturation. A, siCDC5L interfering efficiency by RT-qPCR. B, CDC5L immunostaining after siCDC5L microinjection. C, GVBD rates of oocytes after 24 h IVM were decreased after siCDC5L microinjection. D, cell cycle of oocytes after 44 h IVM was perturbed by siCDC5L microinjection. E, anti-CDC5L antibody injection into GV oocytes significantly decreased the GVBD rates of oocytes after 24 h IVM. F, anti-CDC5L antibody injected into GV oocyte-blocked meiotic resumption, induced abnormal chromatin, and decreased oocyte maturation to MII stage. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Scale bar, 50 μm.