Figure 5.

Hypothyroidism does not affect the postnatal conversion of tau isoforms. A, immunoblots of tau in the cerebellum of control (Cont) and hypothyroid (Hypo) mice from P5 to P32 with Tau5 (upper), RD3 (middle), and RD4 (lower). The amounts of tau were adjusted with Tau5. Rec-mtau is recombinant mouse tau (1N4R, 0N4R, and 0N3R). B, immunoblots of dephosphorylated tau with Tau5. The extracts of cerebellum of control (Cont) and hypothyroid (Hypo) mice from P5 to P21 were treated with ALP as described under “Experimental procedures.” Each isoform of 0N3R, 0N4R, 1N4R, and 2N4R is indicated at the right side of the control blot. The lower panel is the quantification of 0N3R and 0N4R: white circles, control 0N3R; black circles, hypothyroid 0N3R; white squares, control 0N4R; black squares, hypothyroid 0N4R. The data are presented as the mean (n = 3). Error bars represent S.E. a.u., arbitrary units.