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. 2017 Dec 1;293(5):1781–1793. doi: 10.1074/jbc.M117.798918

Figure 6.

Figure 6.

Hypothyroidism delays the transition of the phosphorylation of tau from high to low. A, immunoblots of tau in control (Cont) and hypothyroid (Hypo) mice from P5 to P21 with RD3 after Phos-tag SDS-PAGE. Rec-mtau is recombinant mouse tau. The position of recombinant 0N3R tau is indicated by dotted lines. B, immunoblots of tau in control (Cont) and hypothyroid (Hypo) mice from P5 to P21 with AT8 (upper) and Tau1 (lower) antibodies. The lower panel shows quantifications: white circles, AT8 of control; black circles, AT8 of hypothyroid; white squares, Tau-1 of control; black squares, Tau-1 of hypothyroid. The data are presented as the mean (n = 3; ***, p < 0.001). Error bars represent S.E.; a.u., arbitrary units. C, phosphorylation of tau at Ser-202 and Thr-205. Tau in cerebellum of control (Cont) and hypothyroid (Hypo) mice from P5 to P21 was blotted with anti-phospho-Ser-202 (pS202; CP13) and anti-phospho-Thr-205 (pT205). Quantification is shown to the right: white, control; black, MMI-treated mice. The data are presented as the mean (n = 3; ***, p < 0.001). Error bars represent S.E. D, immunoblots of tau in cerebral cortex of control (Cont) and hypothyroid (Hypo) with anti-phospho-Ser-199 (pS199) and anti-phospho-Ser-422 (pS422) (19). E, immunoblots of tau in cerebral cortex of control (Cont) and hypothyroid (Hypo) mice from P5 to P21 with anti-phospho-Thr-231 (pT231), anti-phospho-Ser-396 (pS396), and anti-phospho-Ser-404 (pS404).