Table 1.
Anti-tau antibodies used in this study
P-tau, phosphorylated tau; dep-tau, dephosphorylated tau; Mono, monoclonal; Poly, polyclonal; Thermo Sci, Thermo Fisher Scientific.
| Antibody | Specificity | Phosphorylation sites | Type | Source (catalog no., lot no.) |
|---|---|---|---|---|
| AT8 | P-tau | Ser-202/Thr-205 | Mono | Thermo Sci (MN1020, RG2218673) |
| CP13 | P-tau | Ser-202 | Poly | Peter Davies |
| HT-7 | Human tau | Mono | Thermo Sci (MN1000, OB183715) | |
| RD3 | 3R tau | Mono | Millipore (05-803, 2712326) | |
| RD4 | 4R tau | Mono | Millipore (05-804, 2950020) | |
| Tau-1 | dep-tau | Ser-195/198/199/202 | Mono | Millipore (MAB3420, 2370726) |
| Tau5 | Tau | Mono | Millipore (577801, D00139295) | |
| Tau12 | Human tau | Mono | Millipore (MAB2241/2842158) | |
| pS199 | P-tau | Ser-199 | Poly | Abcam (ab81268, GR97739-8) |
| pT205 | P-tau | Thr-205 | Poly | Biosource (44-738Z, 44738-1BA) |
| pT231 | P-tau | Thr-231 | Poly | Abcam (ab151559, GR112038-27) |
| pS396 | P-tau | Ser-396 | Poly | Abcam (ab109390, YG120402C) |
| pS404 | P-tau | Ser-404 | Poly | Abcam (ab30666,GR55583-2) |
| pS422 | P-tau | Ser-422 | Poly | Yasuo Ihara (19) |