Figure 10.

Impact of the F45βY mutation on the lower affinity T1-WT TCR. A, yeast-displayed T1 TCR, F45βY mutant, T1-WT TCR, and T1-WT-F45βY mutant were stained with varying concentrations of tetrameric MART-1·HLA-A2 prepared with streptavidin-PE. Resultant MFU were plotted against ligand concentration to obtain binding curves. B, yeast-displayed T1 TCR, F45βY mutant, T1-WT TCR, and T1-WT-F45βY mutant were stained with varying concentrations of monomeric MART-1·HLA-A2 (biotinylated), followed by streptavidin-PE. Resultant MFU were plotted against ligand concentration to obtain binding curves. Binding curves of T1 and F45βY are shown in the inset.