Figure 11.

Structures of T1 and F45βY mutant TCR modeled by RosettaBackrub. A, selected residues at the Vα/Vβ interface in Mel5 TCR (PDB code 3HG1) were mutated to residues in T1 or F45βY (Fig. S1). Using the RosettaBackrub server (http://kortemmelab.ucsf.edu/backrub),³ models were generated. Overlaid models of T1 (Vα (yellow) and Vβ (cyan)) and F45βY (Vα (red) and Vβ (blue)) are shown. MART-1 (green) is shown in complex with HLA-A2 (gray). B, zoomed-in view of A. Polar interactions between Tyr^45β and Leu^96α and Tyr^45β and Tyr^35β are shown by black, dashed lines.