Figure 5.

Sequence fitness landscape of T1 TCR based on selection of single-codon libraries with anti-human Vβ16 antibody. DNA isolated from T1-SCL (unselected or sorted with anti-human Vβ16) was subjected to deep sequencing. Enrichment or depletion of individual substitutions after sorting was calculated relative to the unselected libraries as a log₂ ratio. Enrichment ratios so obtained were plotted on a color-coded scale ranging from ≤2⁻³ (orange) to ≥2⁵ (blue). Residues in Vα and Vβ are shown in A and B, respectively. *, stop codon.