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Comparison plots of individual substitutions from different sorting conditions of T1 TCR single-codon libraries. Enrichment ratios for each substitution in residues in the CDR, interface, and surface FR when T1-SCL was sorted with different ligands are compared in scatter plots shown in A–C. R2 values for the best-fit line are shown. Monomer, monomeric MART-1·HLA-A2; Tetramer, tetrameric MART-1·HLA-A2.
