Figure 7.

Flow cytometric analysis of T1 TCR and T1 interface libraries for binding to monomeric MART-1·HLA-A2. T1 TCR (A) or interface libraries (B) were expressed on yeast surface and stained with 10 nm monomeric MART-1·HLA-A2, with a prior incubation in PBS or human serum supplemented with 10 mm EDTA (Serum) at 37 °C for 30 min. Change in binding was recorded as a decrease in fluorescence when yeasts expressing T1 or interface libraries were pretreated with serum. After sorting interface libraries twice with 1 nm MART-1·HLA-A2, population of yeast cells displaying interface libraries were obtained that bound with high affinity to MART-1·HLA-A2 without (C) or with prior incubation in serum (D).