Figure 8.

Flow cytometric analysis of Vα/Vβ interface mutants of T1 TCR for binding with peptide·HLA-A2 monomers or tetramers. A, yeast-displayed T1 TCR or interface mutants (F91βY, F45βY/M97αR, F45βY/M97αQ/K41αA, and F45βY) were stained with varying concentrations of monomeric MART-1·HLA-A2 (biotinylated), followed by streptavidin-PE. Resultant MFU were plotted against ligand concentration to obtain dissociation constants for each mutant (listed in Table 1). B, peptide specificity of T1 or interface mutants was determined by staining with 50 nm peptide·HLA-A2 tetramers prepared with streptavidin-PE. bHLA-A2, biotinylated HLA-A2.