Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 18;115(5):E866–E875. doi: 10.1073/pnas.1714622115

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published under the PNAS license.

PMC Copyright notice

Fig. 1. — Activity and analysis of the latent GDF8 prodomain complex. (A) HEK293 (CAGA)₁₂ cells treated with latent GDF8 prodomain complex (GDF8^L; black), acid-activated (GDF8^AA; orange), and free mature (GDF8^apo; green) ligand. Experiments were performed at least twice with each data point measured in triplicate. Shown is a representative experiment. Data were fit by nonlinear regression to a variable slope to determine the EC₅₀. (B) Activity measurement following 1-h incubation of purified GDF8^L (40 nM) at the indicated pH, followed by neutralization before exogenous administration to HEK293 (CAGA)₁₂ cells. Data are shown as the mean ± SEM. (C) SEC analysis of GDF8^L, GDF8^AA, and reformed (GDF8^R) prodomain:ligand complexes. (Inset) The protein composition of the peak visualized by SDS/PAGE under nonreducing conditions.