Fig. 4.

Effect of oxidative stress on TXNIP levels. (A) Serum-starved HEK293 cells were treated with H₂O₂ (100, 200, 300 mM) or left unstimulated. Lysates (100 μg) were analyzed by Western blotting for TXNIP and tubulin levels. (B) Effect of oxidative stress on TXNIP levels in LS-derived and control lymphoblastoids. Four individual LS and three control lymphoblastoid cell lines were treated with 300 mM of H₂O₂ or left unstimulated. Cells were harvested after 2 h and levels of TXNIP mRNA were measured by RT-QPCR. A value of 1 was given to TXNIP mRNA levels in untreated cells (solid bars). (C) Serum-starved HEK293 cells were treated with H₂O₂ (100 mM) or IGF1 (50 ng/mL) or both for 2 h. Lysates (100 μg) were analyzed by Western blotting. (D) Densitometric analysis of Western blot shown in Fig. 4C.